NTSR1 ELISA Kit (Mouse) (OKCD08263)

Catalog No: OKCD08263

Size:96WELLS

NTSR1 ELISA Kit (Mouse) (OKCD08263) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurotensin Receptor 1, High Affinity (NTSR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurotensin Receptor 1, High Affinity (NTSR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurotensin Receptor 1, High Affinity (NTSR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neurotensin Receptor 1, High Affinity (NTSR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurotensin Receptor 1, High Affinity (NTSR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurotensin Receptor 1, High Affinity (NTSR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • NTSR1 Microplate: 96 Wells (12 x 8 Well strips)
  • NTSR1 Lyophilized Standard: 2
  • 100X NTSR1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.066ng/mL

Target Info

• Gene Symbol: NTSR1
• Gene Full Name: neurotensin receptor 1
• Alias Symbols: Nt, NT-, NTR, NTR1, NT-1R, NTR-1
• NCBI Gene Id: 18216
• Protein Name: neurotensin receptor type 1
• Description of Target: G-protein coupled receptor for the tridecapeptide neurotensin (nts). signaling is effected via g proteins that activate a phosphatidylinositol-calcium second messenger system. signaling leads to the activation of downstream map kinases and protects cells against apoptosis.
• Uniprot ID: O88319
• Protein Accession #: NP_061236.1
• Nucleotide Accession #: NM_018766.2