Nts ELISA Kit (Rat) (OKEH04659)

Catalog No: OKEH04659

Size:96T

Rat Nts ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Nts ELISA Kit (Rat) (OKEH04659) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Nts antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Nts and incubated. The found in the sample or standards competes with the biotinylated Nts for limited binding sites on the immobilized anti-Rat Nts antibody. Excess unbound biotinylated Nts and sample or standard Nts is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Nts captured in the well and inversely proportional to the amount of Nts which was contained in the sample or standard.
• ELISA Kit Range: 4.69-300pmol/L
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-Nts Microplate: 96 Wells (12 x 8 Well Strips)
  • Nts Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Nts Biotinylated Complex: 1 x 60 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 2.363pmol/L
• Specificity: Natural and recombinant Rat Neurotensin/neuromedin N
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: NTS
• Gene Full Name: Neurotensin
• NCBI Gene Id: 299757
• Protein Name: P20068
• Description of Target: Neurotensin/neuromedin N
• Uniprot ID: https://www.uniprot.org/uniprot/P20068
• Protein Accession #: NP_001095851.1
• Nucleotide Accession #: NM_001102381.1
• Protein Size (# AA): 169
• Molecular Weight: 20 KDa