NTRK2 Antibody (OAAF00656)

Catalog No: OAAF00656 (Formerly GWB-ASC067)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Trk B Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Trk B.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: Trk B antibody detects endogenous levels of total Trk B protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100
  ELISA: 1:20,000
• Reference: Nakagawara A., Genomics 25:538-546(1995).
  Shelton D.L., J. Neurosci. 15:477-491(1995).
  Allen S.J., Neuroscience 60:825-834(1994)

Target Info

• Gene Symbol: NTRK2
• Gene Full Name: neurotrophic receptor tyrosine kinase 2
• Alias Symbols: BDNF/NT-3 growth factors receptor;BDNF-tropomyosine receptor kinase B;DEE58;EIEE58;GP145-TrkB;neurotrophic tyrosine kinase receptor type 2;OBHD;trk-B;TRKB;TrkB tyrosine kinase;tropomyosin-related kinase B;tyrosine kinase receptor B.
• NCBI Gene Id: 4915
• Protein Name: BDNF/NT-3 growth factors receptor
• Description of Target: Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
• Uniprot ID: Q16620
• Protein Accession #: NP_001007098.1
• Molecular Weight: 91 kDa