Size:96 Wells
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NTN1 ELISA Kit (Mouse) (OKEH03416)

Catalog#: OKEH03416
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Mouse
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology NTN1 ELISA Kit (Mouse) (OKEH03416) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ntn1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ntn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ntn1 captured in well.
Kit Range 31.2 - 2000pg/mL
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Kit Reproducibility Mean Intra-assay CV%: <= 5.1% (n = 20)
Mean Inter-assay CV%: <= 7.6% (n = 20)
Kit Component
Anti-Ntn1 Microplate96 Wells (12 x 8 Well strips)
Ntn1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Ntn1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity 15.66 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Mouse Netrin-1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol NTN1
Official Gene Full Name netrin 1
Alias Symbols AI561871, BC019633, Netrin-1
NCBI Gene Id 18208
Protein Name Netrin-1
Description of Target Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis.
Swissprot Id O09118
Protein Accession # NP_032770.2
Nucleotide Accession # NM_008744.2
Protein Size (# AA) 604
Molecular Weight 68 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Ntn1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Ntn1.
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