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NTH E.Coli Protein (OOPA00142)

2ug
$100.00
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Gene Symbol:
NTH
Alias Symbols:
DNA-(apurinic or apyrimidinic site) lyase, b1633, JW1625.
Host:
Escherichia Coli.
Product Format:
Sterile Filtered colorless solution.
Purity:
Greater than 85.0% as determined by SDS-PAGE.
Purification:
Greater than 85.0% as determined by SDS-PAGE.
Peptide Sequence:
MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I
Formulation:
The NTH solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT, 0.1mM PMSF and 40% glycerol.
:::
Product Introduction: Nth has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme cuts impaired DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5' from the damaged site. Nth is vital for the restoration of both oxidative DNA damage and spontaneous mutagenic lesions.
Product Description: NTH E.Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 231 amino acids (1-211a.a.) and having a molecular mass of 25.7kDa. The NTH is purified by proprietary chromatographic techniques.
Reconstitution and Storage:
NTH should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Datasheets/Manuals:
Printable datasheet for OOPA00142

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