Catalog No: OOPA00142 (Formerly GWB-BSP260)
Size:2UG
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Datasheets/Manuals | Printable datasheet for OOPA00142 |
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Product Format | Sterile Filtered colorless solution. |
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Host | Escherichia Coli. |
:: | Product Introduction: Nth has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme cuts impaired DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5' from the damaged site. Nth is vital for the restoration of both oxidative DNA damage and spontaneous mutagenic lesions. Product Description: NTH E.Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 231 amino acids (1-211a.a.) and having a molecular mass of 25.7kDa. The NTH is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | NTH should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 85.0% as determined by SDS-PAGE. |
Peptide Sequence | MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I |
Formulation | The NTH solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT, 0.1mM PMSF and 40% glycerol. |
Purification | Greater than 85.0% as determined by SDS-PAGE. |
Gene Symbol | NTH |
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Alias Symbols | DNA-(apurinic or apyrimidinic site) lyase, b1633, JW1625. |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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