NTF3 ELISA Kit (Bovine) (OKEH07295)

Catalog No: OKEH07295

Size:96T

Bovine NTF3 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology NTF3 ELISA Kit (Bovine) (OKEH07295) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NTF3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NTF3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NTF3 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.6% (n = 20)
  Mean Inter-assay CV%: <=12.1% (n = 20)
• ELISA Kit Component:
  • NTF3 Microplate: 96 Wells (12 x 8 Well strips)
  • NTF3 Lyophilized Standard: 2
  • 100X Biotinylated NTF3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Seems to promote the survival of visceral and proprioceptive sensory neurons.
  Subcellular Location: Secreted
  Unigene: Bt.56403
  SMR: Q08DT3
  String: 9913.ENSBTAP00000040469
  KEGG: bta:532393
  Pfam: PF00243
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.15ng/mL
• Specificity: Natural and recombinant Bovine Neurotrophin-3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NTF3
• Gene Full Name: neurotrophin 3
• Alias Symbols: neurotrophin-3;NT-3.
• NCBI Gene Id: 532393
• Protein Name: Q08DT3
• Description of Target: Neurotrophin-3
• Uniprot ID: https://www.uniprot.org/uniprot/Q08DT3
• Protein Accession #: NP_001071456.1
• Nucleotide Accession #: NM_001077988.2