Catalog No: OPPA01817 (Formerly GWB-E6140E)
Size:2UG
Price: $75.00
SKU
OPPA01817
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OPPA01817 |
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Predicted Species Reactivity | Mouse |
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Product Format | Lyophilized from 0.02% TFA. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
Host | E. coli |
Additional Information | Description: Neurotrophin-3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids and having a molecular mass of 13606.29 Dalton. The NT-3 is purified by proprietary chromatographic techniques. |
:: | Product Introduction: NT3 a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. |
Reconstitution and Storage | Reconstitution: It is recommended to reconstitute the lyophilized Neurotrophin-3 in sterile 18MOmega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Storage: Lyophilized NGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution NGF-2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Biological Activity | The activity, as determined by the dose-dependent proliferation of BaF3 cells transfected with the TrkB receptor, is typically in the range of 1-10 ng/ml, corresponding to a specific activity of 100,000-1,000,000 units/mg. |
Peptide Sequence | YAEHKSHRGE YSVCDSESLW VTDKSSAIDI RGHQVTVLGE IKTGNSPVKQ YFYETRCKEA RPVKNGCRGI DDKHWNSQCK TSQTYVRALT SENNKLVGWR WIRIDTSCVC ALSRKIGRT |
Gene Symbol | mNT 3 |
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Alias Symbols | NT, NT-, Nt3, HDNF, Ntf-, NGF-2, Ntf-3, AI316846, AI835689 |
NCBI Gene Id | 18205 |
Protein Name | Neurotrophin-3 |
Uniprot ID | P20181 |
Protein Accession # | NP_001157506.1 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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