NT-proBNP ELISA Kit (Rat) : 96 Wells (OKEH00475)

Catalog No: OKEH00475

Size:96T

Human NT-proBNP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology NT-proBNP ELISA Kit (Rat) (OKEH00475) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for NT-proBNP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NT-proBNP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NT-proBNP captured in the well.
• ELISA Kit Range: 15.6-1000pg/ml
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.7% (n = 20)
  Mean Inter-assay CV%: < 6.9% (n = 24)
• ELISA Kit Component:
  • Anti-NT-proBNP Microplate: 96 Wells (12 x 8 Well strips)
  • NT-proBNP Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X NT-proBNP Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 7.86 pg/mL
• Specificity: Natural and recombinant Rat NT-proBNP
• Publications:

  Allisartan isoproxil attenuates oxidative stress and inflammation through the SIRT1/Nrf2/NF‑κB signalling pathway in diabetic cardiomyopathy rats. Mol Med Rep. 23, 1 (2021). 33495841

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for NT-proBNP ELISA Kit (Rat) : 96 Wells (OKEH00475) on CiteAb

Target Info

• Gene Symbol: NT-PROBNP
• Alias Symbols: BNP, Bnf
• NCBI Gene Id: 25105
• Protein Name: Natriuretic peptides B
• Description of Target: The N-terminal prohormone of brain natriuretic peptide (NT-proBNP) is a N-terminal fragment of brain natriuretic peptide.
• Uniprot ID: P13205