NT-proBNP ELISA Kit (Human) : 96 Wells (OKCA00041)

Catalog No: OKCA00041

Size:96 Wells

Human NT-proBNP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Human Serum (n=4)	1:1	95%	80-100%
  Human Serum (n=4)	1:2	97%	91-110%
  Human Serum (n=4)	1:4	93%	85-95%
  Human Serum (n=4)	1:8	95%	90-100%

• ELISA Kit Principle: Aviva Systems Biology NT-proBNP ELISA Kit (Human) (OKCA00041) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for human NT-proBNP has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for human NT-proBNP is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human NT-proBNP captured in well.
  
• ELISA Kit Range: 0.312 ng/mL - 20 ng/mL
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (ng/ml)	2.071	2.226	2.29	2.108	2.022	2.086
  SD	0.081	0.083	0.065	0.088	0.076	0.077
  CV%	7.913	7.511	5.709	8.465	7.591	7.462

• ELISA Kit Component:
  • Anti- NT-proBNP Microplate: 96 Well (12 x 8 Well Strips)
  • NT-proBNP Standard (Lyophilized): 2 x 20 ng
  • 100X Biotinylated Anti- NT-proBNP Detector Antibody: 1 x 120 uL
  • 100X HRP-Avidin: 1 x 120 uL
  • Detection Antibody Diluent: 1 x 15 mL
  • HRP-Avidin Diluent: 1 x 15 mL
  • Sample Diluent: 1 x 50 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: < 0.216 ng/ml
• Specificity: This assay has high sensitivity and excellent specificity for detection of human
  NT-proBNP. No significant cross-reactivity or interference between human
  NT-proBNP and analogues was observed.
  Note: Limited by current skills and knowledge, it is impossible for us to complete
  the cross-reactivity detection between human NT-proBNP and all the analogues,
  therefore, cross reaction may still exist.
• Publications:

  Analysis of NT-proBNP and uric acid due to left ventricle hypertrophy in the patients of aortic valve disease. Pak J Med Sci. 35, 183-188 (2019). 30881420

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for NT-proBNP ELISA Kit (Human) : 96 Wells (OKCA00041) on CiteAb

Target Info

• Gene Symbol: NT-proBNP
• Alias Symbols: BNP, Iso-ANP
• NCBI Gene Id: 4879
• Protein Name: Natriuretic peptides B
• Uniprot ID: P16860