NSUN2 Antibody - C-terminal region (ARP48811_P050)

Catalog No: ARP48811_P050

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. An 85 kDa isoform also contains the peptide sequence. Protein may be modified by phosphorylation.

WB Suggested Anti-NSUN2 Antibody Titration: 0.2-1 ug/ml
Positive Control: Hela cell lysate

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NSUN2
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: FINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEP
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-NSUN2 (ARP48811_P050) antibody is Catalog # AAP48811 (Previous Catalog # AAPP28861)
• Enhanced Validation:

  Relative Expression (Western Blot)

• Reference: Huttenhofer,A., (2007) Mol. Biol. Cell 18 (3), 1107-1117
• Publications:

  Stem cell function and stress response are controlled by protein synthesis. Nature. 534, 335-40 (2016). 27306184

Target Info

• Gene Symbol: NSUN2
• Gene Full Name: NOP2/Sun domain family, member 2
• Alias Symbols: MISU, MRT5, SAKI, TRM4
• NCBI Gene Id: 54888
• Protein Name: tRNA (cytosine(34)-C(5))-methyltransferase
• Description of Target: Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).[supplied by OMIM].
• Uniprot ID: Q08J23
• Protein Accession #: NP_060225
• Nucleotide Accession #: NM_017755
• Protein Size (# AA): 767
• Molecular Weight: 86 kDa
• Protein Interactions: SUMO2; SUMO3; UBC; SUMO1; NEDD8; TP53RK; PUS1; EFTUD1; XPO5; UGGT1; OSGEP; PANK4; THG1L; SH3GLB1; KIAA1279; UBXN7; TTLL12; ARPC2; ACTR2; ACTR3; FUBP1; TSN; SH3GL1; RPA1; RDX; RANGAP1; PPP2CB; PFDN5; PFDN1; NRD1; PPP1R12A; HARS; GTF2E1; CALU; CALR; UBD; VC