Catalog No: OKCD00877 (Formerly GWB-KBBBU3)
Size:96WELLS
Price: $675.00
SKU
OKCD00877
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Datasheets/ManualsPrintable datasheet for NSF ELISA Kit (Human) (OKCD00877)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Ethylmaleimide Sensitive Factor (NSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of N-Ethylmaleimide Sensitive Factor (NSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-Ethylmaleimide Sensitive Factor (NSF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-Ethylmaleimide Sensitive Factor (NSF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NSF Microplate96 Wells (12 x 8 Well Strips)
NSF Lyophilized Standard2 x 20 ng
100X Biotinylated NSF Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.117 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of N-Ethylmaleimide Sensitive Factor (NSF). No significant cross-reactivity or interference between N-Ethylmaleimide Sensitive Factor (NSF) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNSF
Gene Full NameN-ethylmaleimide sensitive factor, vesicle fusing ATPase
Alias SymbolsDEE96;epididymis secretory sperm binding protein;NEM-sensitive fusion protein;N-ethylmaleimide-sensitive factor-like protein;N-ethylmaleimide-sensitive fusion protein;SEC18;SKD2;vesicle-fusing ATPase;vesicular-fusion protein NSF.
NCBI Gene Id4905
Protein NameVesicle-fusing ATPase
Description of TargetRequired for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).
Uniprot IDP46459
Protein Accession #NP_006169
Nucleotide Accession #NM_006178.3
Protein Size (# AA)744
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