NSD2 Antibody - N-terminal region (ARP33489_P050)

Catalog No: ARP33489_P050

WB Suggested Anti-WHSC1 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that WHSC1 is expressed in HepG2

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human WHSC1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 93%; Guinea Pig: 88%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 76%; Rat: 86%
• Peptide Sequence: Synthetic peptide located within the following region: KYNVGDLVWSKVSGYPWWPCMVSADPLLHSYTKLKGQKKSARQYHVQFFG
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-NSD2 (ARP33489_P050) antibody is Catalog # AAP33489 (Previous Catalog # AAPP04539)
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that WHSC1 is expressed in HepG2

• Reference: Santra,M., et al., (2003) Blood 101 (6), 2374-2376
• Publications:

  Li, J. et al. Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas. Neuro. Oncol. 10, 45-51 (2008). 18182627

Target Info

• Gene Symbol: NSD2
• Gene Full Name: nuclear receptor binding SET domain protein 2
• Alias Symbols: WHS, TRX5, KMT3F, KMT3G, MMSET, WHSC1, REIIBP
• NCBI Gene Id: 7468
• Protein Name: histone-lysine N-methyltransferase NSD2
• Description of Target: This gene encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences.
• Uniprot ID: O96031
• Protein Accession #: NP_055734
• Nucleotide Accession #: NM_014919
• Protein Size (# AA): 802
• Molecular Weight: 88kDa
• Protein Interactions: HIST1H3A; HIST3H3; HDAC1; TRIM28; UBC; SF3A1; HNRNPR; MPHOSPH10; RAD50; SART3; NUP93; GTF3C4; NOLC1; DDX21; BAZ1B; DDX18; KHSRP; RRP1; SMARCA5; SMC1A; RBM10; XRCC5; VIM; EZR; TOP2B; TOP1; TMPO; SMARCA4; SFPQ; RPN1; RBBP4; RANGAP1; PTBP1; PRKDC; PLRG1; PKP