NRPS1 Recombinant Protein (Aspergillus fumigatus) (OPCA04487)

Catalog No: OPCA04487

Product Info

• Predicted Species Reactivity: Aspergillus fumigatus|Neosartorya fumigata
• Product Format: Lyophilized 20mM Tris-HCl, 0.5M NaCl, 6% Trehalose
• Additional Information: Species Specificity Detail: eosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
• Reconstitution and Storage: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/ml. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Store working aliquots at 4°C for up to one week. Avoid repeated freeze/thaw cycles.
• Formulation: Affinity purified using IMAC
• Purity: Greater than 90% as determined by SDS-PAGE.
• Protein Sequence: Partial Protein: LSPIQKLHFMVRKEGQGYFNQSVVTRIDRQINDQDMRRAVEAVVMRHSMLRSRLVDPSTGNSLQLRITEDVAGSYRWRTHYMTAQNEIENAIAESQLCINAFVGPVFAVDFCYVDEDSHNLLSLVAHHLVVDIVSWRIILEDLEDFLLNPQGFVLQNSSLPFQTWCRLQDEQCESVAFENDVQLEDLPAPDLAYWGMEHRQMTYGDVICETFELDPGSTQSILLECHQSLRTEPVDLFLAALVHSFGQTFPERTLPVIYNEGHGREVWDSSLDISRTVGWFTTLYPIFVQEIVSEDPARTVARVKDLRRQVSDNGRQKFASRMFTGKGQQTCRHHYPLEMTFNYVGQHRDLQKQDGLFQLMGHMAGEAGQGGGAADFGEETPRFALLEISALVVQGQLRFTFSFNRFMRHQSGIHAWISRCHQLLASLGQKLQSLAPQPTLSDFPMLSL
• Source: E.coli
• Protein Range: 894-1342 aa
• Tag: N-terminal 6xHis-SUMO-tagged
• Reference: Phylogenomic analysis of non-ribosomal peptide synthetases in the genus Aspergillus.Cramer R.A. Jr., Stajich J.E., Yamanaka Y., Dietrich F.S., Steinbach W.J., Perfect J.R.Gene 383:24-32(2006)

Target Info

• Gene Symbol: AFUA_1G10380
• Gene Full Name: nonribosomal peptide synthase Pes1
• Alias Symbols: Afu1g10380;AFUA_1G10380;nonribosomal peptide synthase Pes1.
• NCBI Gene Id: 3510659
• Protein Name: Nonribosomal peptide synthetase 1
• Description of Target: Nonribosomal peptide synthesis (NRPS) is a key mechanism responsible for the biosynthesis of bioactive metabolites which are potentially contributing to organismal virulence. Contributes to improved fungal tolerance against oxidative stress, during the infection process.
• Uniprot ID: Q4WT66
• Protein Accession #: XP_752404
• Nucleotide Accession #: XM_747311
• Protein Size (# AA): Recombinant
• Molecular Weight: 64.2 kDa