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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OASE00308-FITC |
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Predicted Species Reactivity | Mouse, Rat |
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Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
Clonality | Monoclonal |
Clone | S364-51 |
Isotype | IgG2a |
Host | Mouse |
Conjugation | FITC: Fluorescein Isothiocyanate |
Application | WB, ICC |
Additional Information | Background Info: Detects a 160 kDa protein. |
:: | Scientific Background: Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2. |
:: | Certificate of Analysis: 1ug/mL was sufficient for detection of NrCAM in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | Fusion protein amino acids 30-845 (extracellular domain) of mouse NrCAM (accession no. Q810U4) Rat: 96% identity (795/822 amino acids identical) Human: 91% identity (753/822 amino acids identical) ~50% identity with Neurofascin |
Purification | Protein G Purified |
Concentration | 1 mg/ml |
Dilution | 1:1000 WB |
Storage Buffer | PBS pH 7.4, 50% glycerol, 0.1% sodium azide |
Gene Symbol | Nrcam |
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Gene Full Name | neuron-glia-CAM-related cell adhesion molecule |
Alias Symbols | B, Bravo, mKIAA0343, C030017F07Rik, C130076O07Rik |
NCBI Gene Id | 319504 |
Uniprot ID | Q810U4 |
Protein Accession # | NP_001139503.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "NrCAM Antibody : FITC (OASE00308-FITC)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse, Rat".
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How long will it take to receive "NrCAM Antibody : FITC (OASE00308-FITC)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "NrCAM Antibody : FITC (OASE00308-FITC)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "NrCAM Antibody : FITC (OASE00308-FITC)"?
This target may also be called "B, Bravo, mKIAA0343, C030017F07Rik, C130076O07Rik" in publications.
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What is the shipping cost for "NrCAM Antibody : FITC (OASE00308-FITC)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "NrCAM Antibody : FITC (OASE00308-FITC)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "NrCAM Antibody : FITC (OASE00308-FITC)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "NrCAM Antibody : FITC (OASE00308-FITC)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "NRCAM"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "NRCAM"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "NRCAM"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "NRCAM"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "NRCAM"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "NRCAM"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.