Catalog No: OKCD00926 (Formerly GWB-KBBRG5)
Size:96WELLS
Price: $675.00
SKU
OKCD00926
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Datasheets/ManualsPrintable datasheet for NR5A2 ELISA Kit (Human) (OKCD00926)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Liver Receptor Homolog 1 (LRH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Liver Receptor Homolog 1 (LRH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Liver Receptor Homolog 1 (LRH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Liver Receptor Homolog 1 (LRH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Liver Receptor Homolog 1 (LRH1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Liver Receptor Homolog 1 (LRH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NR5A2 Microplate96 Wells (12 x 8 Well Strips)
NR5A2 Lyophilized Standard2 x 20 ng
100X Biotinylated NR5A2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.112 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Liver Receptor Homolog 1 (LRH1). No significant cross-reactivity or interference between Liver Receptor Homolog 1 (LRH1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNR5A2
Gene Full Namenuclear receptor subfamily 5 group A member 2
Alias SymbolsAlpha-1-fetoprotein transcription factor;B1-binding factor;b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus;B1F;B1F2;CPF;CYP7A promoter-binding factor;fetoprotein-alpha 1 (AFP) transcription factor;FTF;FTZ-F1;FTZ-F1beta;hB1F-2;Hepatocytic transcription factor;hepatocytic transcription factor hB1F-3;liver nuclear receptor homolog-1 variant 2;Liver receptor homolog 1;LRH1;LRH-1;nuclear receptor subfamily 5 group A member 2.
NCBI Gene Id2494
Protein NameNuclear receptor subfamily 5 group A member 2
Description of TargetNuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (PubMed:20159957). Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
Uniprot IDO00482
Protein Accession #NP_001263393
Nucleotide Accession #NM_001276464.1
Protein Size (# AA)541
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