NR4A1 ELISA Kit (Rat) (OKCD08575)

Catalog No: OKCD08575

Size:96WELLS

NR4A1 ELISA Kit (Rat) (OKCD08575) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nerve Growth Factor IB (NGFIB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nerve Growth Factor IB (NGFIB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nerve Growth Factor IB (NGFIB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nerve Growth Factor IB (NGFIB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nerve Growth Factor IB (NGFIB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nerve Growth Factor IB (NGFIB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • NR4A1 Microplate: 96 Wells (12 x 8 Well strips)
  • NR4A1 Lyophilized Standard: 2
  • 100X NR4A1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.058ng/mL

Target Info

• Gene Symbol: Nr4a1
• Gene Full Name: nuclear receptor subfamily 4, group A, member 1
• Alias Symbols: HMR;immediate early gene transcription factor NGFI-B;nerve growth factor induced protein I-B;Nerve growth factor-induced protein I-B;Ngfi-b;nuclear receptor subfamily 4 group A member 1;Nur77;Orphan nuclear receptor HMR.
• NCBI Gene Id: 79240
• Protein Name: Nuclear receptor subfamily 4 group A member 1
• Description of Target: Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (By similarity).
• Uniprot ID: P22829
• Protein Accession #: NP_077364.2
• Nucleotide Accession #: NM_024388.2