NR4A1 ELISA Kit (Human) (OKEH08254)

Catalog No: OKEH08254

Size:96T

Human NR4A1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology NR4A1 ELISA Kit (Human) (OKEH08254) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NR4A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NR4A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NR4A1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.6% (n = 20)
  Mean Inter-assay CV%: <=8.3% (n = 20)
• ELISA Kit Component:
  • NR4A1 Microplate: 96 Wells (12 x 8 Well strips)
  • NR4A1 Lyophilized Standard: 2
  • 100X Biotinylated NR4A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation.
  Subcellular Location: Cytoplasm Nucleus
  Unigene: Hs.524430
  SMR: P22736
  String: 9606.ENSP00000353427
  KEGG: hsa:3164
  MIM: 139139
  Pfam: PF00104
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.073 ng/mL
• Specificity: Natural and recombinant Human Nuclear receptor subfamily 4 group A member 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NR4A1
• Gene Full Name: nuclear receptor subfamily 4 group A member 1
• Alias Symbols: early response protein NAK1;GFRP1;growth factor-inducible nuclear protein N10;HMR;hormone receptor;N10;NAK-1;nerve growth factor IB nuclear receptor variant 1;NGFIB;NP10;nuclear hormone receptor NUR/77;nuclear receptor subfamily 4 group A member 1;NUR77;orphan nuclear receptor HMR;orphan nuclear receptor TR3;ST-59;steroid receptor TR3;testicular receptor 3;TR3;TR3 orphan receptor.
• NCBI Gene Id: 3164
• Protein Name: P22736
• Description of Target: Nuclear receptor subfamily 4 group A member 1
• Uniprot ID: https://www.uniprot.org/uniprot/P22736
• Protein Accession #: NP_001189162.1
• Nucleotide Accession #: NM_001202233.1