NR3C2 ELISA Kit (Human) (OKCD00242)

Catalog No: OKCD00242 (Formerly GWB-KBBKJ9)

Size:96WELLS

Human NR3C2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mineralocorticoid Receptor (MR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mineralocorticoid Receptor (MR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mineralocorticoid Receptor (MR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mineralocorticoid Receptor (MR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mineralocorticoid Receptor (MR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mineralocorticoid Receptor (MR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NR3C2 Microplate: 96 Wells (12 x 8 Well Strips)
  • NR3C2 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated NR3C2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mineralocorticoid Receptor (MR). No significant cross-reactivity or interference between Mineralocorticoid Receptor (MR) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: NR3C2
• Gene Full Name: nuclear receptor subfamily 3 group C member 2
• Alias Symbols: aldosterone receptor;MCR;mineralocorticoid receptor;mineralocorticoid receptor 1;mineralocorticoid receptor 2;mineralocorticoid receptor delta;MLR;MR;NR3C2VIT;Nuclear receptor subfamily 3 group C member 2;nuclear receptor subfamily 3, group C, member 2 variant 3.
• NCBI Gene Id: 4306
• Protein Name: Mineralocorticoid receptor
• Description of Target: Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
• Uniprot ID: P08235
• Protein Accession #: NP_000892
• Nucleotide Accession #: NM_000901.4
• Protein Size (# AA): 984