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Catalog No: OKEH02850
Size:96T
Price: $525.00
SKU
OKEH02850
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NR3C1 ELISA Kit (Human) : 96 Wells (OKEH02850)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology NR3C1 ELISA Kit (Human) (OKEH02850) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human NR3C1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated NR3C1 and incubated. The NR3C1 found in the sample or standards competes with the biotinylated NR3C1 for limited binding sites on the immobilized anti-human NR3C1 antibody. Excess unbound biotinylated NR3C1 and sample or standard NR3C1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated NR3C1 captured in the well and inversely proportional to the amount of NR3C1 which was contained in the sample or standard.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-NR3C1 Microplate96 Wells (12 x 8 Well strips)
NR3C1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X NR3C1-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity23 pg/mL
SpecificityNatural and recombinant Human Glucocorticoid receptor
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolNR3C1
Gene Full Namenuclear receptor subfamily 3 group C member 1
Alias SymbolsGCCR;GCR;GCRST;glucocorticoid nuclear receptor variant 1;glucocorticoid receptor;GR;GRL;Nuclear receptor subfamily 3 group C member 1;nuclear receptor subfamily 3 group C member 1 variant hGR-B(54);nuclear receptor subfamily 3 group C member 1 variant hGR-B(77);nuclear receptor subfamily 3 group C member 1 variant hGR-B(93);nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor).
NCBI Gene Id2908
Protein NameP04150
Description of TargetGlucocorticoid receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P04150
Protein Accession #NP_000167.1
Nucleotide Accession #NM_000176.2
Protein Size (# AA)777
Molecular Weight85 kDa
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