Catalog No: OCOA01463
Size:20UG
Price: $179.00
SKU
OCOA01463
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NR2F6 Protein Lysate (OCOA01463)

Datasheets/ManualsPrintable datasheet for NR2F6 Protein Lysate (OCOA01463)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionNR2F6 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolNR2F6
Gene Full Namenuclear receptor subfamily 2 group F member 6
Alias SymbolsEAR2;EAR-2;ERBAL2;ERBA-related gene-2;nuclear receptor subfamily 2 group F member 6;nuclear receptor V-erbA-related;v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 2;V-erbA-related protein 2.
NCBI Gene Id2063
Protein NameF1D8R3, NM_005234, NP_005225, P10588
Description of TargetHomo sapiens nuclear receptor subfamily 2 group F member 6 (NR2F6), mRNA, Nuclear receptor subfamily 2 group F member 6, nuclear receptor subfamily 2 group F member 6 [Homo sapiens], Nuclear receptor V-erbA-related
Uniprot IDhttps://www.uniprot.org/uniprot/P10588
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_005225
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_005234
Molecular Weight42.8 kDa
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