NR1I3 ELISA Kit (Rat) (OKCD08126)

Catalog No: OKCD08126

Size:96WELLS

NR1I3 ELISA Kit (Rat) (OKCD08126) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Constitutive Androstane Receptor (CAR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Constitutive Androstane Receptor (CAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Constitutive Androstane Receptor (CAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Constitutive Androstane Receptor (CAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Constitutive Androstane Receptor (CAR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Constitutive Androstane Receptor (CAR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • NR1I3 Microplate: 96 Wells (12 x 8 Well strips)
  • NR1I3 Lyophilized Standard: 2
  • 100X NR1I3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.060ng/mL

Target Info

• Gene Symbol: Nr1i3
• Gene Full Name: nuclear receptor subfamily 1, group I, member 3
• Alias Symbols: CAR;constitutive androstane receptor;nuclear receptor constitutive active receptor;nuclear receptor subfamily 1 group I member 3;strain Fischer nuclear receptor (CAR).
• NCBI Gene Id: 65035
• Protein Name: Nuclear receptor subfamily 1 group I member 3
• Description of Target: Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element (By similarity).
• Uniprot ID: Q9QUS1
• Protein Accession #: NP_075230.1
• Nucleotide Accession #: NM_022941.4