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Catalog No: OKCD02803
Size:96WELLS
Price: $725.00
SKU
OKCD02803
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Datasheets/ManualsPrintable datasheet for NR1I2 ELISA Kit (Rat) (OKCD02803)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnane X Receptor (PXR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pregnane X Receptor (PXR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pregnane X Receptor (PXR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pregnane X Receptor (PXR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pregnane X Receptor (PXR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pregnane X Receptor (PXR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NR1I2 Microplate96 Wells (12 x 8 Well strips)
NR1I2 Lyophilized Standard2
100X Biotinylated NR1I2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.113 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Pregnane X Receptor (PXR). No significant cross-reactivity or interference between Pregnane X Receptor (PXR) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolNr1i2
Gene Full Namenuclear receptor subfamily 1, group I, member 2
Alias Symbolsnuclear receptor subfamily 1 group I member 2;nuclear receptor subfamily 1, group 1, member 2;orphan nuclear receptor PXR;Pregnane X receptor;pregnane X receptor (nuclear receptor sub family 1, group I, member 2);PXR.
NCBI Gene Id84385
Protein NameNuclear receptor subfamily 1 group I member 2
Description of TargetNuclear receptor that binds and is activated by a variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, endogenous compounds and drugs. Response to specific ligands is species-specific, due to differences in the ligand-binding domain. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes (By similarity).
Uniprot IDQ9R1A7
Protein Accession #NP_443212.1
Nucleotide Accession #NM_052980.2
Protein Size (# AA)431
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