- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
NR1I2 Antibody - N-terminal region : Biotin (ARP36016_P050-Biotin)
Datasheets/Manuals | Printable datasheet for anti-NR1I2 (ARP36016_P050-Biotin) antibody |
---|
Predicted Species Reactivity | Human |
---|---|
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Biotin |
Application | IHC, WB |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human NR1I2 |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human: 100% |
Peptide Sequence | Synthetic peptide located within the following region: KKEMIMSDEAVEERRALIKRKKSERTGTQPLGVQGLTEEQRMMIRELMDA |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-NR1I2 (ARP36016_P050-Biotin) antibody is Catalog # AAP36016 (Previous Catalog # AAPP07326) |
Reference | Hesselink,D.A., (2008) Pharmacogenomics 9 (6), 783-789 |
Publications | Sivertsson, L., Edebert, I., Palmertz, M. P., Ingelman-Sundberg, M. & Neve, E. P. A. Induced CYP3A4 expression in confluent Huh7 hepatoma cells as a result of decreased cell proliferation and subsequent pregnane X receptor activation. Mol. Pharmacol. 83, 659-70 (2013). WB, IHC, Human 23264496 Dou, W. et al. Chrysin ameliorates chemically induced colitis in the mouse through modulation of a PXR/NF-κB signaling pathway. J. Pharmacol. Exp. Ther. 345, 473-82 (2013). WB, IHC, Human 23536316 |
Gene Symbol | NR1I2 |
---|---|
Gene Full Name | Nuclear receptor subfamily 1, group I, member 2 |
Alias Symbols | BXR, PAR, PRR, PXR, SAR, SXR, ONR1, PAR1, PAR2, PARq |
NCBI Gene Id | 8856 |
Protein Name | Nuclear receptor subfamily 1 group I member 2 |
Description of Target | NR1I2 belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. NR1I2 contains a zinc finger domain.NR1I2 is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. NR1I2 belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized. |
Uniprot ID | O75469 |
Protein Accession # | NP_003880 |
Nucleotide Accession # | NM_003889 |
Protein Size (# AA) | 434 |
Molecular Weight | 50kDa |
Protein Interactions | NCOA1; RPS6KB1; UBC; RBBP7; DDB1; UBR5; DYRK2; NCOR2; SDF4; NCOA3; TFAP2C; MAPK7; NFATC4; HSP90AA1; FGR; ATP6AP1; RXRA; RBCK1; NCOA2; SUMO1; SUMO2; SUMO3; NCOA6; SRC; RXRG; RXRB; CHMP1A; NUCB2; ACTN2; PPARGC1A; EIF3I; TADA3; NCOR1; PRMT1; NR1I2; PSMC5; PO |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "NR1I2 Antibody - N-terminal region : Biotin (ARP36016_P050-Biotin)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
-
How long will it take to receive "NR1I2 Antibody - N-terminal region : Biotin (ARP36016_P050-Biotin)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
-
What buffer format is "NR1I2 Antibody - N-terminal region : Biotin (ARP36016_P050-Biotin)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "NR1I2 Antibody - N-terminal region : Biotin (ARP36016_P050-Biotin)"?
This target may also be called "BXR, PAR, PRR, PXR, SAR, SXR, ONR1, PAR1, PAR2, PARq" in publications.
-
What is the shipping cost for "NR1I2 Antibody - N-terminal region : Biotin (ARP36016_P050-Biotin)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "NR1I2 Antibody - N-terminal region : Biotin (ARP36016_P050-Biotin)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "NR1I2 Antibody - N-terminal region : Biotin (ARP36016_P050-Biotin)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "50kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "NR1I2 Antibody - N-terminal region : Biotin (ARP36016_P050-Biotin)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "NR1I2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "NR1I2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "NR1I2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "NR1I2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "NR1I2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "NR1I2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.