Catalog No: OKDD00433 (Formerly GWB-KBBZA6)
Size:96 Wells
Price: $725.00
SKU
OKDD00433
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- $55: Antibody & Protein in US
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NPTX1 ELISA Kit (Human) (OKDD00433)
Datasheets/Manuals | Printable datasheet for NPTX1 ELISA Kit (Human) (OKDD00433) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||||
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Application | Enzyme-linked immunosorbent assay | ||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology NPTX1 ELISA Kit (Human) (OKDD00433) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for NPTX1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPTX1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NPTX1 captured in well. | ||||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NPTX1 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NPTX1 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV<10%
Inter-Assay: CV<12% | ||||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell lysates | ||||||||||||||||||||||||
Sensitivity | < 0.049 ng/mL | ||||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of NPTX1. No significant cross-reactivity or interference between NPTX1 and analogues was observed. | ||||||||||||||||||||||||
Assay Info | Quantitative Sandwich ELISA | ||||||||||||||||||||||||
Description |
Gene Symbol | NPTX1 |
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Gene Full Name | neuronal pentraxin 1 |
Alias Symbols | neuronal pentraxin I;neuronal pentraxin-1;NP1;NP-I. |
NCBI Gene Id | 4884 |
Protein Name | Neuronal pentraxin-1 |
Description of Target | May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses. |
Uniprot ID | Q15818 |
Protein Accession # | NP_002513.2 |
Nucleotide Accession # | NM_002522.3 |
Protein Size (# AA) | 432 |
Molecular Weight | 47 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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