Size:96 Wells
$519.00
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SKU
OKEH03551
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NPS ELISA Kit (Rat) (OKEH03551)

Catalog#: OKEH03551
Domestic: within 1 week delivery ,International: 1 week
More Information
Predicted Species Reactivity Rat
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology NPS ELISA Kit (Rat) (OKEH03551) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Nps antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Nps and incubated. The Nps found in the sample or standards competes with the biotinylated Nps for limited binding sites on the immobilized anti-human Nps antibody. Excess unbound biotinylated Nps and sample or standard Nps is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Nps captured in the well and inversely proportional to the amount of Nps which was contained in the sample or standard.
Kit Range 0.156 - 10ng/mL
Kit Component
ComponentAmount
Anti-Nps Microplate96 Wells (12 x 8 Well strips)
Nps Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Nps-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity 0.082 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Rat Neuropeptide S
Assay Info Assay Methodology: Quantitative Competitive ELISA
Gene Symbol NPS
Official Gene Full Name neuropeptide S
NCBI Gene Id 100360071
Protein Name Neuropeptide S
Description of Target May play an important anorexigenic role. Modulates arousal and anxiety as well as increases locomotor activity. Binds to its receptor NPSR1 with nanomolar affinity to increase intracellular calcium concentrations.
Swissprot Id P0C0P7
Protein Accession # NP_001258042.1
Nucleotide Accession # NM_001271113.1
Protein Size (# AA) 89
Molecular Weight 10 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Nps.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Nps.
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