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Catalog No: OCOA12057
Size:20UG
Price: $179.00
SKU
OCOA12057
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NPRL2 Protein Lysate (OCOA12057)

Datasheets/ManualsPrintable datasheet for NPRL2 Protein Lysate (OCOA12057)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionNPRL2 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolNPRL2
Gene Full NameNPR2 like, GATOR1 complex subunit
Alias Symbols2810446G01Rik;FFEVF2;G21 protein;GATOR complex protein NPRL2;gene 21 protein;homologous to yeast nitrogen permease (candidate tumor suppressor);nitrogen permease regulator 2-like protein;NPR2;NPR2L;NPR2-like protein;tumor suppressor candidate 4;TUSC4.
NCBI Gene Id10641
Protein NameNM_006545, NP_006536, Q8WTW4
Description of TargetGATOR complex protein NPRL2, GATOR complex protein NPRL2 [Homo sapiens], Homo sapiens NPR2 like, GATOR1 complex subunit (NPRL2), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q8WTW4
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_006536
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_006545
Molecular Weight43.7 kDa
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