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Catalog No: OKCD00892 (Formerly GWB-KBBWR3)
Size:96WELLS
Price: $725.00
SKU
OKCD00892
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Datasheets/ManualsPrintable datasheet for NPR3 ELISA Kit (Rat) (OKCD00892)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Natriuretic Peptide Receptor 3 (NPR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Natriuretic Peptide Receptor 3 (NPR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Natriuretic Peptide Receptor 3 (NPR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Natriuretic Peptide Receptor 3 (NPR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Natriuretic Peptide Receptor 3 (NPR3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Natriuretic Peptide Receptor 3 (NPR3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Npr3 Microplate96 Wells (12 x 8 Well Strips)
Npr3 Lyophilized Standard2 x 20 ng
100X Biotinylated Npr3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.115 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Natriuretic Peptide Receptor 3 (NPR3). No significant cross-reactivity or interference between Natriuretic Peptide Receptor 3 (NPR3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNpr3
Gene Full Namenatriuretic peptide receptor 3
Alias SymbolsANP-C;ANP-CR;ANPRC;ANPR-C;atrial natriuretic peptide clearance receptor;Atrial natriuretic peptide clearence receptor 3;atrial natriuretic peptide C-type receptor;atrial natriuretic peptide receptor 3;atrial natriuretic peptide receptor type C;natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C);NPRC;NPR-C.
NCBI Gene Id25339
Protein NameAtrial natriuretic peptide receptor 3
Description of TargetReceptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
Uniprot IDP41740
Protein Accession #NP_000899
Nucleotide Accession #NM_000908.3
Protein Size (# AA)535
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