Catalog No: OKCD06726
Size:96WELLS
Price: $850.00
SKU
OKCD06726
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for NPPC ELISA Kit (Mouse) (OKCD06726)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Natriuretic Peptide (CNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Natriuretic Peptide (CNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Natriuretic Peptide (CNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C-Type Natriuretic Peptide (CNP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Type Natriuretic Peptide (CNP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Type Natriuretic Peptide (CNP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
NPPC Microplate96 Wells (12 x 8 Well strips)
NPPC Lyophilized Standard2
100X NPPC HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 12.2pg/mL
Gene SymbolNppc
Gene Full Namenatriuretic peptide type C
Alias SymbolsC;CNP;C-type natriuretic peptide;lb;lbab;natriuretic peptide precursor C;natriuretic peptide precursor type C.
NCBI Gene Id18159
Protein NameC-type natriuretic peptide
Description of TargetHormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3.
Uniprot IDQ61839
Protein Accession #NP_035063.1
Nucleotide Accession #NM_010933.5
Write Your Own Review
You're reviewing:NPPC ELISA Kit (Mouse) (OKCD06726)
Your Rating
We found other products you might like!