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Catalog No: OKEH03865
Size:96T
Price: $625.00
SKU
OKEH03865
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology NPPC ELISA Kit (Bovine) (OKEH03865) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-bovine NPPC antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated NPPC and incubated. The NPPC found in the sample or standards competes with the biotinylated NPPC for limited binding sites on the immobilized anti-bovine NPPC antibody. Excess unbound biotinylated NPPC and sample or standard NPPC is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated NPPC captured in the well and inversely proportional to the amount of NPPC which was contained in the sample or standard.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit Component
ComponentAmount
Anti-NPPC Microplate96 Wells (12 x 8 Well strips)
NPPC Lyophilized Standard2
Sample Diluent1 x 20 mL
100X NPPC-Biotin Complex1 x 60 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity13 pg/mL
SpecificityNatural and recombinant Bovine C-type natriuretic peptide
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolNPPC
Gene Full Namenatriuretic peptide C
Alias SymbolsC-type natriuretic peptide;natriuretic peptide precursor C;SVSP15.
NCBI Gene Id281356
Protein NameP55206
Description of TargetC-type natriuretic peptide
Uniprot IDhttps://www.uniprot.org/uniprot/P55206
Protein Accession #NP_776550.1
Nucleotide Accession #NM_174125.2
Protein Size (# AA)126
Molecular Weight13 kDa
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