NPPA ELISA Kit (Mouse) (OKCD01191)

Catalog No: OKCD01191 (Formerly GWB-KBBKL4)

Size:96WELLS

Mouse Nppa ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	78-92%	80-98%	79-93%	79-91%
  EDTA plasma(n=5)	78-97%	84-104%	96-103%	78-102%
  heparin plasma(n=5)	78-93%	86-102%	86-101%	91-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	101
  EDTA plasma(n=5)	95-103	98
  heparin plasma(n=5)	78-96	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Nppa Microplate: 96 Wells (12 x 8 Well Strips)
  • Nppa Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated Nppa Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 29 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP). No significant cross-reactivity or interference between N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Nppa
• Gene Full Name: natriuretic peptide type A
• Alias Symbols: A;Anf;ANP;atrial natriuretic factor prohormone;atrial natriuretic peptide prohormone;atriopeptigen;cardiodilatin;CDD;natriuretic peptide precursor type A;natriuretic peptides A;P;Pnd;preproANF;preproANP;preproCDD-ANF;prepronatriodilatin;proANF;proANP.
• NCBI Gene Id: 230899
• Protein Name: Natriuretic peptides A
• Description of Target: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
• Uniprot ID: P05125
• Protein Accession #: NP_032751
• Nucleotide Accession #: NM_008725.3
• Protein Size (# AA): 152