NPPA ELISA Kit (Dog) (OKEH07351)

Catalog No: OKEH07351

Size:96T

Dog NPPA ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	92-102% (n = 20)	100-111% (n = 20)	102-111% (n = 20)	85-85% (n = 20)
  EDTA plasma(n=5)	88-97% (n = 20)	116-125% (n = 20)	102-113% (n = 20)	107-117% (n = 20)
  heparin plasma(n=5)	88-98% (n = 20)	98-106% (n = 20)	97-106% (n = 20)	93-105% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology NPPA ELISA Kit (Dog) (OKEH07351) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPPA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPPA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NPPA captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=2.9% (n = 20)
  Mean Inter-assay CV%: <=6.5% (n = 20)
• ELISA Kit Component:
  • NPPA Microplate: 96 Wells (12 x 8 Well strips)
  • NPPA Lyophilized Standard: 2
  • 100X Biotinylated NPPA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilatation. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  Subcellular Location: Secreted
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 10.8pg/mL
• Specificity: Natural and recombinant Dog Natriuretic peptides A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NPPA
• Gene Full Name: natriuretic peptide A
• Alias Symbols: ANF;ANP;atrial natriuretic factor prohormone;atrial natriuretic peptide prohormone;atriopeptigen;cardiodilatin;CDD;natriuretic peptide precursor A;natriuretic peptides A;preproANF;preproANP;preproCDD-ANF;prepronatriodilatin;proANF;proANP.
• NCBI Gene Id: 608289
• Protein Name: P07499
• Description of Target: Natriuretic peptides A
• Uniprot ID: https://www.uniprot.org/uniprot/P07499
• Protein Accession #: NP_001300780.1
• Nucleotide Accession #: NM_001313851.1