NPHS2 ELISA Kit (Human) : 96 Wells (OKEH00672)

Catalog No: OKEH00672

Size:96T

Human NPHS2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology NPHS2 ELISA Kit (Human) (OKEH00672) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPHS2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPHS2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NPHS2 captured in well.
  
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.3% (n = 20)
  Mean Inter-assay CV%: < 8.9% (n = 20)
• ELISA Kit Component:
  • Anti-NPHS2 Microplate: 96 Wells (12 x 8 Well strips)
  • NPHS2 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated NPHS2 Detector Antibody: 1 x 120 ul
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Human Podocin
• Publications:

  The Presence of Urinary Renal Progenitor Cells in Stable Kidney Transplant Recipients Anticipates Allograft Deterioration. Front Physiol. 9, 1412 (2018). 30364198

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for NPHS2 ELISA Kit (Human) : 96 Wells (OKEH00672) on CiteAb

Target Info

• Gene Symbol: NPHS2
• Gene Full Name: NPHS2 stomatin family member, podocin
• Alias Symbols: nephrosis 2, idiopathic, steroid-resistant (podocin);NPHS2, podocin;PDCN;podocin;SRN1.
• NCBI Gene Id: 7827
• Protein Name: Q9NP85
• Description of Target: Podocin
• Uniprot ID: https://www.uniprot.org/uniprot/Q9NP85
• Protein Accession #: NP_001284504.1
• Nucleotide Accession #: NM_001297575.1
• Protein Size (# AA): 383
• Molecular Weight: 42 kDa