Catalog No: OKEH08517
Size:96T
Price: $800.00
SKU
OKEH08517
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology NPC2 ELISA Kit (Pig) (OKEH08517) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPC2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NPC2 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 107% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=3.8% (n = 20)
Mean Inter-assay CV%: <=8.1% (n = 20)
ELISA Kit Component
ComponentAmount
NPC2 Microplate96 Wells (12 x 8 Well strips)
NPC2 Lyophilized Standard2
100X Biotinylated NPC2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes (By similarity). NPC2 binds cholesterol with a 1:1 stoichiometry (PubMed:10366780). Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol (By similarity). The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport (By similarity).
::Subcellular Location: Secreted Endoplasmic reticulum Lysosome Interaction with cell-surface M6PR mediates endocytosis and targeting to lysosomes.
::Unigene: Ssc.16778
SMR: O97763
String: 9823.ENSSSCP00000002564
::KEGG: ssc:397410
Pfam: PF02221
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.39ng/mL
SpecificityNatural and recombinant Pig NPC intracellular cholesterol transporter 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNPC2
Gene Full NameNPC intracellular cholesterol transporter 2
Alias Symbols16 kDa secretory protein;16kDa secretory protein;epididymal secretory protein E1;niemann Pick type C2 protein homolog;NPC intracellular cholesterol transporter 2.
NCBI Gene Id397410
Protein NameO97763
Description of TargetNPC intracellular cholesterol transporter 2
Uniprot IDhttps://www.uniprot.org/uniprot/O97763
Protein Accession #NP_999371.1
Nucleotide Accession #NM_214206.1
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