Catalog No: OKEH08517
Size:96T
Price: $800.00
SKU
OKEH08517
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Porcine, Sus scrofa | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology NPC2 ELISA Kit (Pig) (OKEH08517) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPC2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NPC2 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.78-50ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=3.8% (n = 20) Mean Inter-assay CV%: <=8.1% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes (By similarity). NPC2 binds cholesterol with a 1:1 stoichiometry (PubMed:10366780). Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol (By similarity). The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport (By similarity). | ||||||||||||||||||||||
:: | Subcellular Location: Secreted Endoplasmic reticulum Lysosome Interaction with cell-surface M6PR mediates endocytosis and targeting to lysosomes. | ||||||||||||||||||||||
:: | Unigene: Ssc.16778 SMR: O97763 String: 9823.ENSSSCP00000002564 | ||||||||||||||||||||||
:: | KEGG: ssc:397410 Pfam: PF02221 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 0.39ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Pig NPC intracellular cholesterol transporter 2 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | NPC2 |
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Gene Full Name | NPC intracellular cholesterol transporter 2 |
Alias Symbols | 16 kDa secretory protein;16kDa secretory protein;epididymal secretory protein E1;niemann Pick type C2 protein homolog;NPC intracellular cholesterol transporter 2. |
NCBI Gene Id | 397410 |
Protein Name | O97763 |
Description of Target | NPC intracellular cholesterol transporter 2 |
Uniprot ID | https://www.uniprot.org/uniprot/O97763 |
Protein Accession # | NP_999371.1 |
Nucleotide Accession # | NM_214206.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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