NPC1 ELISA Kit (Human) (OKEH08372)

Catalog No: OKEH08372

Size:96T

Human NPC1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology NPC1 ELISA Kit (Human) (OKEH08372) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NPC1 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.4% (n = 20)
  Mean Inter-assay CV%: <=6.7% (n = 20)
• ELISA Kit Component:
  • NPC1 Microplate: 96 Wells (12 x 8 Well strips)
  • NPC1 Lyophilized Standard: 2
  • 100X Biotinylated NPC1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: (Microbial infection) Acts as an endosomal entry receptor for ebolavirus.
  Subcellular Location: Late endosome membrane Multi-pass membrane protein Lysosome membrane Multi-pass membrane protein
  Unigene: Hs.464779
  SMR: O15118
  String: 9606.ENSP00000269228
  KEGG: hsa:4864
  MIM: 257220
  Pfam: PF16414
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 39pg/mL
• Specificity: Natural and recombinant Human NPC intracellular cholesterol transporter 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NPC1
• Gene Full Name: NPC intracellular cholesterol transporter 1
• Alias Symbols: Niemann-Pick C1 protein;NPC;NPC intracellular cholesterol transporter 1;POGZ;SLC65A1;truncated Niemann-Pick C1.
• NCBI Gene Id: 4864
• Protein Name: O15118
• Description of Target: NPC intracellular cholesterol transporter 1
• Uniprot ID: https://www.uniprot.org/uniprot/O15118
• Protein Accession #: NP_000262.2
• Nucleotide Accession #: NM_000271.4