NOX4 ELISA Kit (Rat) (OKCD07802)

Catalog No: OKCD07802

Size:96WELLS

NOX4 ELISA Kit (Rat) (OKCD07802) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADPH Oxidase 4 (NOX4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADPH Oxidase 4 (NOX4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADPH Oxidase 4 (NOX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NADPH Oxidase 4 (NOX4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NADPH Oxidase 4 (NOX4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NADPH Oxidase 4 (NOX4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • NOX4 Microplate: 96 Wells (12 x 8 Well strips)
  • NOX4 Lyophilized Standard: 2
  • 100X NOX4 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.113ng/mL

Target Info

• Gene Symbol: Nox4
• Gene Full Name: NADPH oxidase 4
• Alias Symbols: kidney oxidase-1;kidney superoxide-producing NADPH oxidase;kox-1;NADPH oxidase 4.
• NCBI Gene Id: 85431
• Protein Name: NADPH oxidase 4
• Description of Target: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.
• Uniprot ID: Q924V1
• Protein Accession #: NP_445976.1
• Nucleotide Accession #: NM_053524.1