NOX4 Antibody - C-terminal region (OABB01075)

Catalog No: OABB01075

Size:100UG

Anti-NADPH oxidase 4 antibody, OABB01075, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Cardiac Muscle Tissue Lysate
Lane 3: Rat Spleen Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: 293T Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 7: SMMC Cell Lysate

Anti-NADPH oxidase 4 antibody, OABB01075, IHC(P)
IHC(P): Rat Kidney Tissue

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Flow cytometry|Immunocytochemistry|Immunohistochemistry|Immunohistochemistry-Frozen|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: NADPH oxidase 4 is an enzyme that in humans is encoded by the NOX4 gene, and is a member of the NOX family of NADPH oxidases. This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Rat: Human, Mouse: By Heat
  Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human
  Immunocytochemistry : 0.5-1 ug/ml: Human
  Flow Cytometry: 1-3 ug/1x10^6 cells: Human
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for NADPH oxidase 4(NOX4) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse;Rat.

Target Info

• Gene Symbol: Nox4
• Gene Full Name: NADPH oxidase 4
• Alias Symbols: AI648021;kidney oxidase-1;kidney superoxide-producing NADPH oxidase;KOX-1;NADPH oxidase 4;renal NAD(P)H-oxidase;superoxide-generating NADPH oxidase 4.
• NCBI Gene Id: 50490
• Protein Name: NADPH oxidase 4
• Description of Target: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.
• Uniprot ID: Q9JHI8
• Molecular Weight: 66519 MW