NOX3 ELISA Kit (Mouse) (OKEH08714)

Catalog No: OKEH08714

Size:96T

Mouse NOX3 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology NOX3 ELISA Kit (Mouse) (OKEH08714) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NOX3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NOX3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NOX3 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.8% (n = 20)
  Mean Inter-assay CV%: <=10.2% (n = 20)
• ELISA Kit Component:
  • NOX3 Microplate: 96 Wells (12 x 8 Well strips)
  • NOX3 Lyophilized Standard: 2
  • 100X Biotinylated NOX3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. Plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity.
  Subcellular Location: Membrane Multi-pass membrane protein
  Unigene: Mm.328756
  String: 10090.ENSMUSP00000024565
  KEGG: mmu:224480
  Pfam: PF08022
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.078ng/mL
• Specificity: Natural and recombinant Mouse NADPH oxidase 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Nox3
• Gene Full Name: NADPH oxidase 3
• Alias Symbols: GP91-3;head-tilt;het;NADPH oxidase 3;nmf25;nmf250.
• NCBI Gene Id: 224480
• Protein Name: Q672J9
• Description of Target: NADPH oxidase 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q672J9
• Protein Accession #: NP_945196.2
• Nucleotide Accession #: NM_198958.2