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Catalog No: OABB00620 (Formerly GWB-BBP542)
Size:100UG
Price: $432.00
SKU
OABB00620
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsPrintable datasheet for NOX2 Polyclonal Antibody (OABB00620)
Product Info
Tested Species ReactivityHuman, Rat
Predicted Species ReactivityHamster|Human|Mouse|Rat
Product FormatLyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
ClonalityPolyclonal
ClonePolyclonal
IsotypeRabbit IgG
HostRabbit
ApplicationImmunohistochemistry|Western blot
Additional InformationNotes: Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
::Background: NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species(ROS) in a process dependent on microtubules(X-ROS signaling).
Reconstitution and Storage2°C to 8°C|-20°C
ImmunogenPolypeptide
PurificationAffinity Purified
Predicted Homology Based on Immunogen SequenceHuman; Mouse; Rat
Concentration500 ug/ml
SpecificityNo cross reactivity with other proteins.
Application InfoWestern blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
Reference1. Bionda, C., Li, X. J., van Bruggen, R., Eppink, M., Roos, D., Morel, F., Stasia, M.-J. Functional
analysis of two-amino acid substitutions in gp91phox in a patient with X-linked flavocytochrome
b(558)-positive chronic granulomatous disease by means of transgenic PLB-985 cells. Hum. Genet.
115: 418-427, 2004.
2. Bolscher, B. G. J. M., de Boer, M., de Klein, A., Weening, R. S., Roos, D. Point mutations in the
beta-subunit of cytochrome b(558) leading to X-linked chronic granulomatous disease. Blood 77:
2482-2487, 1991.
3. "Entrez Gene: CYBB cytochrome b-245, beta polypeptide (chronic granulomatous disease)"
Storage BufferEach vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Publications

Different signalling in infarcted and non-infarcted areas of rat failing hearts: A role of necroptosis and inflammation. J Cell Mol Med. 23, 6429-6441 (2019). 31328381

Description
Gene SymbolCYBB
Gene Full Namecytochrome b-245 beta chain
Alias SymbolsAMCBX2;CGD;CGD91-phox;CGDX;cytochrome b(558) subunit beta;cytochrome b-245 beta polypeptide;cytochrome b-245 heavy chain;cytochrome b558 subunit beta;GP91-1;GP91PHOX;GP91-PHOX;heme-binding membrane glycoprotein gp91phox;IMD34;NADPH oxidase 2;neutrophil cytochrome b 91 kDa polypeptide;NOX2;p22 phagocyte B-cytochrome;p91-PHOX;superoxide-generating NADPH oxidase heavy chain subunit.
NCBI Gene Id1536
Protein NameCytochrome b-245 heavy chain
Description of TargetCritical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Uniprot IDP04839
Molecular Weight65336 MW
  1. What is the species homology for "NOX2 Polyclonal Antibody (OABB00620)"?

    The tested species reactivity for this item is "Human, Rat". This antibody is predicted to have homology to "Hamster|Human|Mouse|Rat".

  2. How long will it take to receive "NOX2 Polyclonal Antibody (OABB00620)"?

    This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".

  3. What buffer format is "NOX2 Polyclonal Antibody (OABB00620)" provided in?

    This item is provided in "Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "NOX2 Polyclonal Antibody (OABB00620)"?

    This target may also be called "AMCBX2;CGD;CGD91-phox;CGDX;cytochrome b(558) subunit beta;cytochrome b-245 beta polypeptide;cytochrome b-245 heavy chain;cytochrome b558 subunit beta;GP91-1;GP91PHOX;GP91-PHOX;heme-binding membrane glycoprotein gp91phox;IMD34;NADPH oxidase 2;neutrophil cytochrome b 91 kDa polypeptide;NOX2;p22 phagocyte B-cytochrome;p91-PHOX;superoxide-generating NADPH oxidase heavy chain subunit." in publications.

  5. What is the shipping cost for "NOX2 Polyclonal Antibody (OABB00620)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "NOX2 Polyclonal Antibody (OABB00620)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "NOX2 Polyclonal Antibody (OABB00620)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "65336 MW".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "NOX2 Polyclonal Antibody (OABB00620)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "CYBB"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CYBB"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CYBB"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CYBB"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CYBB"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CYBB"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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