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Catalog No: OKEH01864
Size:96T
Price: $600.00
SKU
OKEH01864
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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NOX1 ELISA Kit (Human) : 96 Wells (OKEH01864)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology NOX1 ELISA Kit (Human) (OKEH01864) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NOX1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NOX1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NOX1 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
NOX1 Microplate96 Wells (12 x 8 Well strips)
NOX1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PRKCE Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.31 ng/mL
SpecificityNatural and recombinant Human NADPH oxidase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNOX1
Gene Full NameNADPH oxidase 1
Alias SymbolsGP91-2;mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating);mitogenic oxidase 1;MOX1;NADH/NADPH mitogenic oxidase subunit P65-MOX;NADPH oxidase 1;NADPH oxidase homolog-1;NOH1;NOH-1.
NCBI Gene Id27035
Protein NameQ9Y5S8
Description of TargetNADPH oxidase 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Y5S8
Protein Accession #NP_001258744.1
Nucleotide Accession #NM_001271815.1
Protein Size (# AA)564
Molecular Weight65 kDa
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