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Catalog No: OKCA02437
Size:96 Wells
Price: $754.00
SKU
OKCA02437
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat109104-113
1:29389-99
1:410096-104
1:88884-92
ELISA Kit PrincipleAviva Systems Biology Notum ELISA Kit (Mouse) (OKCA02437) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Notum has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Notum is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Notum captured in the well.
ELISA Kit Range0.156 - 10 ng/mL
ELISA Kit Recovery
Sample TypeMean Recovery (%)Range (%)
Serum (n=5)8581-89
EDTA Plasma (n=4)9894-102
ELISA Kit ReproducibilityMean Intra-Assay: CV <12% (n=20)
Mean Inter-Assay: CV <15% (n=24)
ELISA Kit Component
ComponentAmount
Notum Microplate96 Wells (12 x 8 Well strips)
Notum Lyophilized Standard2
100X Biotinylated Notum Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates
Sensitivity0.039 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNOTUM
Alias Symbols5730593N15Rik
NCBI Gene Id77583
Protein NamePalmitoleoyl-protein carboxylesterase NOTUM
Description of TargetCarboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleylation of WNT proteins. Serine palmitoleylation of WNT proteins is required for efficient binding to frizzled receptors.
Uniprot IDQ8R116
Protein Accession #NP_780472
Nucleotide Accession #NM_175263
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