NOTCH4 ELISA Kit (Human) (OKCD09282)

Catalog No: OKCD09282

Size:96WELLS

NOTCH4 ELISA Kit (Human) (OKCD09282) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Notch Homolog 4 (NOTCH4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Notch Homolog 4 (NOTCH4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Notch Homolog 4 (NOTCH4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Notch Homolog 4 (NOTCH4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Notch Homolog 4 (NOTCH4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Notch Homolog 4 (NOTCH4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • NOTCH4 Microplate: 96 Wells (12 x 8 Well strips)
  • NOTCH4 Lyophilized Standard: 2
  • 100X NOTCH4 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.058ng/mL

Target Info

• Gene Symbol: NOTCH4
• Gene Full Name: notch receptor 4
• Alias Symbols: INT3;neurogenic locus notch homolog protein 4;notch 4;Notch homolog 4.
• NCBI Gene Id: 4855
• Protein Name: Neurogenic locus notch homolog protein 4
• Description of Target: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity).
• Uniprot ID: Q99466
• Protein Accession #: NP_004548.3
• Nucleotide Accession #: NM_004557.3