- NCBI Gene Id:
- Protein Name:
- Neurogenic locus notch homolog protein 2
- Alias Symbols:
- hN2, AGS2, NOTCH2
- Replacement Item:
- This antibody may replace item sc-32344 from Santa Cruz Biotechnology.
- Description of Target:
- RABBIT ANTI HUMAN NOTCH 2
- Protein Size (# AA):
- ELISA, WB
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express NOTCH2.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express NOTCH2.
- The immunogen for anti-NOTCH2 antibody: synthetic peptide corresponding to amino acids 2396-2409 of human Notch 2.
- Predicted Species Reactivity:
- Predicted Homology Based on Immunogen Sequence:
- Product Format:
- Serum - liquid
- Reconstitution and Storage:
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for anti-NOTCH2 antibody - OASA08044
- NOTCH 2
- Polyclonal IgG
- Application Info:
- ELISA: 1/30000 - 1/90000
Western Blotting: 1/400 - 1/2000
- Preservative Stabilisers: 0.09% - Sodium Azide (NaN3)
Antiserum Preparation: Antisera to Notch 2 were raised by repeated immunisations of rabbits with highly purified antigen.
- Buffer Solutions: Phosphate buffered saline pH7.2
- Target Reference:
- 1. McDaniell, R. et al. (2006) NOTCH2 mutations cause Alagille syndrome, a heterogeneous disorder of the notch signaling pathway. Am. J. Hum. Genet. 79: 169-173.
2. Witt, C.M. et al. (2003) Activated Notch2 potentiates CD8 lineage maturation and promotes the selective development of B1 B cells. Mol. Cell. Biol. 23: 8637-8650.
3. Saito, T. et al. (2003) Notch2 is preferentially expressed in mature B cells and indispensable for marginal zone B lineage development. Immunity. 18: 675-685.
4. Hubmann, R. et al. (2002) Notch2 is involved in the overexpression of CD23 in B-cell chronic lymphocytic leukemia. Blood. 99: 3742-3747.
5. Shimizu, K. et al. (2002) Functional diversity among Notch1, Notch2, and Notch3 receptors. Biochem. Biophys. Res. Commun. 291: 775-779.