Notch1 Antibody : Biotin (OASE00278-Biotin)

Catalog No: OASE00278-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: S253-32
• Isotype: IgG1
• Host: Mouse
• Conjugation: Biotin
• Application: WB, ICC
• Additional Information: Background Info: Detects >270kDa, ~120kDa and small fragments due to proteolysis. Does not cross-react with Notch 2 or Notch3.
  Scientific Background: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
  Certificate of Analysis: 1ug/mL was sufficient for detection of Notch1 in 20ug of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 20-216(extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1, accession number Q01705.
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 WB
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Swiatek P.J., et al. (1994) Genes Dev. 8: 707-719. 2. Simpson P. (1994) The Notch Receptors. Austin, TX: R.G. Landes Company.
  3. Lindsell C.E., et al. (1995) Cell 80: 909-917.
  4. Uyttendaele H., et al. (1996) Development 122:
  2251-2259.
  5. Girard L., et al. (1996) Genes Dev. 10: 1930-1944.
  

Target Info

• Gene Symbol: Notch1
• Gene Full Name: notch 1
• Alias Symbols: N, Mi, N1, Ta, lin, Mis6, Tan1, lin-12, 9930111A19Rik
• NCBI Gene Id: 18128
• Uniprot ID: Q01705
• Protein Accession #: NP_032740.3