Notch 2 Recombinant Antibody [B6] (OAAV00640)

Catalog No: OAAV00640

Size:200UG

Immunofluorescence staining of fixed HeLa cells with anti-Notch 2 antibody B6 (OAAV00640) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B6 (OAAV00640) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom OAAV00640, DAPI, merged channels and a negative control.

Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B6

Product Info

• Tested Species Reactivity: Human
  Mouse
• Predicted Species Reactivity: Human
  Mouse
• Product Format: PBS with 0.02% Proclin 300.
• Clonality: Recombinant Monoclonal
• Clone: B6
• Isotype: IgG, kappa
• Host: Rabbit
• Application: Blocking,
  Enzyme-linked immunosorbent assay,
  Immunofluorescence
• Reconstitution and Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Immunogen: NRR of mouse Notch 2.
• Purification: Purified
• Specificity: An engineered ScFv fragment antibody that binds to both mouse mNRR2 and human hNRR2, but not NRR1.
• Description: This chimeric rabbit antibody was made using the variable domain sequences of the original human scFv format, for improved compatibility with existing reagents, assays and techniques.

Target Info

• Gene Symbol: NOTCH2
• Gene Full Name: notch receptor 2
• Alias Symbols: AGS2;HJCYS;hN2;neurogenic locus notch homolog protein 2;notch 2;Notch homolog 2.
• NCBI Gene Id: 4853
• Protein Name: Neurogenic locus notch homolog protein 2
• Description of Target: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed:29149593). Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
• Uniprot ID: Q04721
• Protein Accession #: NP_001186930.1
• Nucleotide Accession #: NM_001200001.1