NOS3 ELISA Kit (Guinea pig) (OKCA01052)

Catalog No: OKCA01052

Size:96 Wells

Product Info

• Predicted Species Reactivity: Guinea Pig
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology NOS3 ELISA Kit (Guinea pig) (OKCA01052) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NOS3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NOS3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NOS3 captured in the well.
• ELISA Kit Range: 500 - 8,000 pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01052 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01052 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • NOS3 Microplate: 96 Wells (12 x 8 Well strips)
  • NOS3 Lyophilized Standard: 2 x 8 ng
  • 100X Biotinylated NOS3 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 250 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: NOS3
• Gene Full Name: Nitric oxide synthase, endothelial
• Alias Symbols: ECNOS, eNOS, nitric oxide synthase 3
• Protein Name: Nitric oxide synthase, endothelial
• Description of Target: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
• Uniprot ID: P97270
• Protein Accession #: NP_001166456.1
• Nucleotide Accession #: NM_001172985.1