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Datasheets/Manuals | Printable datasheet for NOS3 Antibody (OAAF01173) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human eNOS. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | eNOS (Ab-1179) antibody detects endogenous levels of total eNOS protein. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10,000 |
Reference | OLIVIER FERON. Ann. N.Y. Acad. Sci., Jun 1999; 874: 11. Siripan Phattanarudee. FASEB J, Mar 2006; 20: A1387. Sci. STKE, Sep 2004; 2004: tw318. |
Gene Symbol | NOS3 |
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Gene Full Name | nitric oxide synthase 3 |
Alias Symbols | cNOS;constitutive NOS;ECNOS;EC-NOS;endothelial NOS;eNOS;nitric oxide synthase 3 (endothelial cell);nitric oxide synthase 3 transcript variant eNOS-delta20;nitric oxide synthase 3 transcript variant eNOS-delta20-21;nitric oxide synthase 3 transcript variant eNOS-delta21;nitric oxide synthase, endothelial;NOS type III;NOSIII. |
NCBI Gene Id | 4846 |
Protein Name | Nitric oxide synthase, endothelial |
Description of Target | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. |
Uniprot ID | P29474 |
Protein Accession # | NP_000594.2 |
Molecular Weight | 133 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "NOS3 Antibody (OAAF01173)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "NOS3 Antibody (OAAF01173)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "NOS3 Antibody (OAAF01173)" provided in?
This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "NOS3 Antibody (OAAF01173)"?
This target may also be called "cNOS;constitutive NOS;ECNOS;EC-NOS;endothelial NOS;eNOS;nitric oxide synthase 3 (endothelial cell);nitric oxide synthase 3 transcript variant eNOS-delta20;nitric oxide synthase 3 transcript variant eNOS-delta20-21;nitric oxide synthase 3 transcript variant eNOS-delta21;nitric oxide synthase, endothelial;NOS type III;NOSIII." in publications.
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What is the shipping cost for "NOS3 Antibody (OAAF01173)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "NOS3 Antibody (OAAF01173)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "NOS3 Antibody (OAAF01173)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "133 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "NOS3 Antibody (OAAF01173)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "NOS3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "NOS3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "NOS3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "NOS3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "NOS3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "NOS3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.