NOS2 ELISA Kit (Monkey) (OKCA01610)

Catalog No: OKCA01610

Size:96 Wells

Monkey NOS2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Monkey
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology NOS2 ELISA Kit (Monkey) (OKCA01610) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NOS2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NOS2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NOS2 captured in the well.
• ELISA Kit Range: 1.25 - 80 IU/mL
• ELISA Kit Component:
  • NOS2 Microplate: 96 Wells (12 x 8 Well strips)
  • NOS2 Lyophilized Standard: 2 x 80 IU
  • 100X Biotinylated NOS2 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 0.4 IU/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: NOS2
• Gene Full Name: Nitric oxide synthase, inducible
• Alias Symbols: HEP-NOS, INOS, NOS, NOS2A, NOS, type II|nitric oxide synthase 2A|nitric oxide synthase 2A (inducible, hepatocytes)|nitric oxide synthase, macrophage
• Protein Name: Nitric oxide synthase, inducible
• Description of Target: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.
• Uniprot ID: O46660
• Protein Accession #: XP_014974372.1
• Nucleotide Accession #: XM_015118886.1