Norrin Antibody (OAPB00865)

Catalog No: OAPB00865

Size:100UG

Click here to learn more about Aviva's By-Request Conjugation Service.

Western blot analysis of Norrin in Jurkat cell lysate with Norrin antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry of Norrin in Jurkat cells with Norrin antibody at 5 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse
• Product Format: Liquid. Norrin Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: Norrin antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Norrin antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human Norrin. The immunogen is located within the first 50 amino acids of Norrin.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-Norrin antibody is Catalog # OEPB02985
• Application Info: Norrin antibody can be used for detection of Norrin by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Other Applications Image 1 Data: Immunocytochemistry of Norrin in Jurkat cells with Norrin antibody at 5 ug/mL.
• Reference: Bergen W, Meindl A, van de Pol TJ, et al. Isolation of a candidate gene for Norrie disease by positional cloning. Nat. Genet.1992; 1:199-203.;Meitinger T, Meindl A, Bork P, et al. Molecular modelling of the Norrie disease protein predicts a cysteine knot growth factor tertiary structure. Nat. Genet.1993; 5:376-80.;Xu Q, Wang Y, Dabdoub A, et al. Vascular development in the retina and inner ear: control by Norrin and Frizzled-4, a high-affinity ligand-receptor pair. Cell2004; 116:883-95.;Ohlmann A, Scholz M, Goldwich A, et al. Ectopic norrin induces growth of ocular capillaries and restores normal retinal angiogenesis. J. Neurosci.2005; 25:1701-10.

Target Info

• Gene Symbol: NDP
• Gene Full Name: norrin cystine knot growth factor NDP
• Alias Symbols: EVR2;FEVR;ND;NDP, norrin cystine knot growth factor;Norrie disease (pseudoglioma);norrie disease protein;norrin;X-linked exudative vitreoretinopathy 2 protein.
• NCBI Gene Id: 4693
• Protein Name: Norrin|norrin precursor [Homo sapiens]
• Description of Target: Activates the canonical Wnt signaling pathway through FZD4 and LRP5 coreceptor. Plays a central role in retinal vascularization by acting as a ligand for FZD4 that signals via stabilizing beta-catenin (CTNNB1) and activating LEF/TCF-mediated transcriptional programs. Acts in concert with TSPAN12 to activate FZD4 independently of the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1). May be involved in a pathway that regulates neural cell differentiation and proliferation. Possible role in neuroectodermal cell-cell interaction.
• Uniprot ID: Q00604
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_000257
• Molecular Weight: Predicted: 26 kDa
  Observed: 28 kDa