NORMAL GOAT SERUM (NGS) (OORA01661)

Catalog No: OORA01661

Size:10ML

Product Info

• Product Format: Lyophilized 0.02M Potassium Phosphate, 0.15M Sodium Chloride (pH 7.2) with 0.01% (w/v) sodium azide and 0.01% (w/v) gentamicin sulfate
• Conjugation: Unconjugated
• Application: Chromatin Immunoprecipitation, Enzyme-linked immunosorbent assay, Flow cytometry, Fluorescence-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Sodium sodecyl sulfate - polyacrylamide gel electrophoresis, Western blot
• Reconstitution and Storage: Store Normal Goat Serum (NGS) at 4°C prior to restoration. Restore with 10 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze Goat Serum at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Concentration: 105 mg/ml
• Application Info: Goat Serum blocking reagent or NGS is ideal for blocking procedures such as WB, ELISA and immunochemistry to prevent nonspecific binding.
• Purification: Goat Serum blocking reagent or NGS was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. Normal Goat Serum was obtained from non-immunized healthy goats.

Target Info

• Alias Symbols: blocking goat serum, blocking grade, 10% NGS
• Description of Target: Normal Goat Serum can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications.