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Catalog No: OKEH02565
Size:96T
Price: $700.00
SKU
OKEH02565
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02565
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Noradrenaline ELISA Kit (OKEH02565) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Noradrenaline antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Noradrenaline and incubated. The Noradrenaline found in the sample or standards competes with the biotinylated Noradrenaline for limited binding sites on the immobilized anti-Noradrenaline antibody. Excess unbound biotinylated Noradrenaline and sample or standard Noradrenaline is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Noradrenaline captured in the well and inversely proportional to the amount of Noradrenaline which was contained in the sample or standard.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Noradrenaline Microplate96 Wells (12 x 8 Well strips)
Noradrenaline Lyophilized Standard2 x 2,000 pg
Sample Diluent1 x 20 mL
100X Noradrenaline-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: NA
Target Name: Noradrenaline
::Pubchem: 439260
::Chemical formula: C8H11NO3
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity9.8 pg/mL
SpecificityNatural and recombinant General Noradrenaline
Publications

A Mouse Homolog of a Human TP53 Germline Mutation Reveals a Lipolytic Activity of p53. Cell Rep. 30, 783-792.e5 (2020). 31968253

The Histamine 3 Receptor Is Expressed in the Heart and Its Activation Opposes Adverse Cardiac Remodeling in the Angiotensin II Mouse Model. Int J Mol Sci. 21, (2020). 33371319

Assay InfoAssay Methodology: Quantitative Competitive ELISA
Description
Alias SymbolsNA, Noradrenaline, NA, NE, Norepinephrine, Isoprenaline
Protein NameNoradrenaline
Description of TargetOne of the most important functions of Noradrenaline is its role as the neurotransmitter released from the sympathetic neurons affecting the heart. An increase in Noradrenaline from the sympathetic nervous system increases the rate of contractions. When Noradrenaline acts as a drug, it increases blood pressure by increasing vascular tone (tension of vascular smooth muscle) through alpha-adrenergic receptor activation; a reflex bradycardia homeostatic baroreflex is overcome by a compensatory reflex preventing an otherwise inevitable drop in heart rate to maintain blood pressure. Noradrenaline is synthesized from dopamine by dopamine beta-hydroxylase.[8] It is released from the adrenal medulla into the blood as a hormone, and is also a neurotransmitter in the central nervous system and sympathetic nervous system, where it is released from noradrenergic neurons in the locus coeruleus. The actions of norepinephrine are carried out via the binding to adrenergic receptors.
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